Advanced Microscopy – Seminar Oct. 30 – Light Sheet Microscopy

A special seminar on Zeiss Lightsheet Z.1 Light Sheet Fluorescence Microscopy (LSFM) is scheduled for Wednesday Oct. 30th at 10:00 a.m. in Room 162 of the Food Safety & Toxicology Bldg. (attached to the south side of the CIPS Bldg.).

This is the second in a series of seminars being sponsored by the Center for Advanced Microscopy on the subject of LSFM.  The Oct. 30th seminar covers the subject of Multi-View LSFM, in which the fluorescence excitation and emission detection are split into two separate light paths, with illumination perpendicular to the detection.  Fluorescence images may be collected faster and with less excitation light than many other fluorescence microscopy techniques, thus permitting optical sectioning and imaging of large samples with very little phototoxicity or photobleaching.

Courtney Akitake from Carl Zeiss Microscopy will be giving the presentation.  The following link provides additional information about the Zeiss Lightsheet Z.1 features and configuration

https://www.zeiss.com/microscopy/us/products/imaging-systems/lightsheet-z-1.html

We hope to see you there.

Michigan Microscopy and Microanalysis Society Meeting Oct. 31

The Center for Advanced Microscopy and MSU are hosting the fall meeting of the Michigan Microscopy and Microanalysis Society at the Kellogg Center on Thursday Oct. 31.

Full information and registration details about the meeting can be found at http://www.michmicroscopy.org

The program includes speakers in biological and physical sciences.  Numerous microscope vendors will be present.

Space is available for volunteered talks and poster presentations.

We hope to see you there.

Advanced Microscopy – Seminar Oct. 8 – Light Sheet Microscopy

A special seminar on Light Sheet Fluorescence Microscopy (LSFM) is scheduled for Tuesday Oct. 8 at 2:00 p.m. in room 162 of the Food Safety & Toxicology Bldg. (attached to the south side of the CIPS Bldg.).

LSFM has much potential in facilitating dynamic live cell microscopy.  The technique has less photobleaching and phototoxicity than conventional confocal microscopy, faster frame rates, and the ability to image deeper within samples.

This is the first in a series of seminars being sponsored by the Center for Advanced Microscopy on the subject of LSFM.  The Oct. 8 seminar covers the subject of Lateral Interference Tilted Excitation (LITE), one of several variations of LSFM.  Chris Baumann from Mizar Imaging will be giving the presentation.  The attached article provides full details of the LITE method.

Please contact Melinda (framem@msu.edu) if you would like to schedule time for a demo of the system or to test your samples on the Tilt LITE module.  Mizar will have a Tilt LITE Module installed in the confocal lab (CIPS room B-7) and will be available for demonstrations Wednesday Oct. 9 and Thursday Oct. 10.

Dates and times of future seminars will be posted.

A New TEM Is Coming To CAM.

A new JEOL 1400 Flash Transmission Electron Microscope (TEM) will be coming to the Center for Advanced (CAM) in the next few months. This new TEM will replace the JEOL 100CX TEM that has served MSU researchers for the past 34 years

1400_Flash

The new JEOL 1400 Flash is at the cutting edge of technology with some very exciting and useful features.

The microscope will be equipped with a newly developed Matataki Flash sCMOS camera that dramatically reduces readout noise (less than 0.9 electrons) while maintaining an extremely high frame rate of 30 frames per second. The camera facilitates another feature, the automated Limitless Panorama Function (automated stitching) that can cover a wide area with no limitation on the number of pixels.

In addition, the microscope has an Optical Microscope Correlation function in which an image from an optical microscope can be imported and overlaid on a TEM image to facilitate correlative microscopy.

The microscope has an easy to use intuitive user interface that should facilitate teaching in NSC-810 and research use.

The lattice resolution is excellent, 0.2 nm using a High Contrast Pole Piece! This resolution will provide outstanding results on all biological samples and will be useful for many physical science TEM users.

The microscope will be equipped with a four grid holder, and all sample holders will be interchangeable between the JEOL 1400 and the other TEM at CAM, the JEOL 2200FS. The stage on the new TEM will be capable of plus or minus 70 degree tilts.

This new microscope represents a major advancement in the TEM capabilities at the Center for Advanced Microscopy and will benefit many MSU researchers and numerous off-campus users.

A Keyence VHX-6000 Digital Microscope Has Arrived At CAM.

A Keyence VHX-6000 microscope is now available at the Center for Advanced Microscopy. This remarkable microscope is the latest development in the field of Digital Microscopy (DM). It makes extensive use of recent breakthroughs in digital cameras, stage micro-motors, advanced zoom lenses, graphics engines, and software.

The microscope takes full Color Images at magnifications from approximately 1X to 5,000X with full Depth-of-Field using the concept of Focus Stacking (Z focus series). The imaging process uses software controlled stage motors and is done automatically. Sample Surface Profiles and 3D information can also be collected. Samples require no processing.

The microscope complements the SEM and CLSM capabilities at CAM. Instruction on the microscope is being incorporated into the SEM class (NSC-820) and the CLSM class (NSC-837). The charges for use of the microscope are posted on the Services page. A Web Page with full information about the microscope will be posted in the near future.

SEM and TEM Photos of the Giant Samba Virus taken at CAM Make the April Cover of Viruses.

Colorized SEM and TEM photos taken at CAM were used for the cover of the April issue of Viruses. The photos are from research being conducted by Kristin Parent and Jason Schrad from the Department of Biochemistry and Molecular Biology.

The images were taken using cryo electron microscopy and ultra-high resolution scanning electron microscopy. Kristin was instrumental in bringing the technique of cryo electron microscopy to Michigan State University.

 

An SEM Photo from CAM Makes the April Cover of Microbial Cell.

A colorized SEM photo taken at CAM was used for the cover of the April issue of Microbial Cell.  The photo is from research by James Kremer and shows the bacterial pathogen Pseudomonas syringae on the leaf of Arabidiopsis thaliana.

James is finishing his Ph.D. (Plant Research Laboratory and Microbiology and Molecular Genetics;  Sheng-Yang He and James Tiedje, co-advisors) and now works for AgBiome, Research Triangle Park, North Carolina.

CAM User Thomas Pinnavaia Honored as an AAAS Fellow.

Thomas Pinnavaia, a user of CAM for many years, was recently named an AAAS Fellow by the American Association for the Advancement of Science. The award was made “For distinguished contributions to the field of inorganic materials chemistry, particularly for innovative synthesis of mesophases via supramolecular assembly and the intercalation of lamellar solids.” We extend our congratulations to Tom.

Other CAM users named as AAAS Fellows in the past include Christoph Benning, Dean Della-Penna, Shelagh Ferguson-Miller, Sheng-Yang He, Ken Keegstra, R. James Kirkpatrick, Richard Lenski, Katherine Osteryoung, Danny Schnell, Thomas Sharkey, Michael Thomashow, James Tiedje, and Jonathan Walton.