Transmission Electron Microscopy (TEM) – Biological Sciences

The Center for Advanced Microscopy (CAM) offers full comprehensive transmission electron microscopy services for the biological sciences including plant and animal samples, bacteria, and pathology specimens.  In addition, full services are provided for materials science research such as polymers and plastics that require sample sectioning.

The primary transmission electron microscope for Biological Sciences TEM at the Center for Advanced Microscopy is a JEOL 1400 Flash.  It has a maximum accelerating voltage of 120 kV, is equipped with a Lanthanum Hexaboride “Lab Six” emitter, and has a lattice resolution 0.2 nm using a high contrast pole piece.

The camera is a newly developed Matataki Flash sCMOS design that dramatically reduces readout noise (less than 0.9 electrons) while maintaining an extremely high frame rate of 30 frames per second.  Other features include Limitless Panorama Function (automated stitching) that can cover a wide area with no limitation on the number of pixels and Optical Microscope Correlation function in which an image from an optical microscope can be imported and overlaid on a TEM image to facilitate correlative microscopy.  The microscope has a four grid holder and a high tilt holder capable of plus or minus 70 degree tilts.  The sample holders are interchangeable between the JEOL 1400 and the other TEM at CAM, the JEOL 2200FS.

This microscope offers excellent imaging capability for all biological samples and also for many physical science samples.  In addition, the JEOL 2200FS ultra-high resolution TEM is available if needed.Alicia_1400

The JEOL 1400 Flash TEM at the Center for Advanced Microscopy.


CAM has a full range of sample preparation equipment for all types of biological samples.  Two modern microtomes are available, including an RMC (Boeckeler Instruments) MTX and an RMC Power Tome XL with cryo-sectioning attachment.  Diamond knives are available for experiences users during business hours.

The RMC MYX ultramicrotome has a 15 nm driven cutting stroke for the sectioning of large specimens and hard materials without chatter

The RMC MYX ultramicrotome has a 15 nm driven cutting stroke for the sectioning of large specimens and hard materials without chatter

 

The RMC PTXL ultramicrotome has patented Power Drive technology. When gravity fails, the power cutting stroke has enough strength to cut through even hard non-homogenous specimens. The PTXl is extremely useful for sectioning polymers and thin films

The RMC PTXL ultramicrotome has patented Power Drive technology. When gravity fails, the power cutting stroke has enough strength to cut through even hard non-homogenous specimens. The PTXl is extremely useful for sectioning polymers and thin film

 

Golgi_2

Golgi apparatus, image taken on the JEOL 1400 Flash TEM at the Center for Advanced Microscopy

CAM offers conventional TEM fixation and embedding procedures.  An Electron Microscopy Sciences model 9000 scientific microwave oven is available to facilitate sample fixation and embedding.  Cryo sectioning is also available.  Of also offer negative staining for particles in suspension (bacteria, viruses, lipids, etc.).  Immunogold labeling procedures are available for specific localization of cell components.  We also offer complete services for sectioning of polymers films and plastics for materials science research.

Paraffin embedding and sectioning, cryostat sectioning, and thick plastic sectioning is available to support confocal scanning laser microscopy (CLSM), laser capture microscopy (LCM), and conventional light microscopy.

We offer the following services:

  • Negative staining for samples in suspension (Bacteria, virus, Lipids, etc.)
  • Sample specific nanoparticle staining
  • Immunogold experiments. Specific fixation and embedding procedures for each individual sample.
  • Thin sectioning of thin polymer films
Additional equipment available:
  • Glass knife maker (GKM, RMC, Boeckeler instruments).
  • Ladd carbon evaporator
  • Polymerization oven

For biological TEM services contact Dr. Alicia Withrow,  517-355-5004, pastorle@msu.edu.

See our Service and User Charges page.

For enrollment in NSC-810 Biological TEM Lab, contact Dr. Alicia Withrow,  517-355-5004, pastorle@msu.edu.  This is a limited enrollment course with specific enrollment procedures.  Please see our Courses page for more information.