CAM has taken delivery on a Leica Stellaris 5 confocal laser scanning microscope (CLSM). This microscope is equipped with newly developed white light laser technology. A sub-nanosecond pulsed IR laser is directed into a photonic crystal fiber to generate coherent white light. Small bands of light are then selected by a tunable acousto-optical filter. Coherent light is available in the range of 488 to 685 nanometers. This allows selection of the precise wavelength needed.
The technology also allows FLIM (fluorescence lifetime imaging microscopy) which produces an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample.
This new microscope complements the existing CLSM capabilities at CAM. More information about the Leica Stellaris 5 microscope will be forthcoming on the CAM web page.