Biological Sciences TEM at the Center for Advanced Microscopy use a JEOL100 CXII (Japan Electron Optics Laboratories). There are two Ultramicrotomes: a MTX and a Power Tome XL (PTXL) (RMC, Boekeler Instruments, Tucson, AZ) with a CR-X cryo- sectioning unit for cryo-ultramicrotomy (RMC, Boekeler Instruments, Tucson, AZ). Diamond knives are available for experienced users during business hours. We offer the following services:

• Conventional fixatives and embedding procedures for ultrastructure studies (Karnovsky fixatives, Spurr, Poly/Bed 812 resins)
• Negative staining for samples in suspension (Bacteria, virus, Lipids, etc.)
• Sample specific nanoparticle staining
• Immunogold experiments. Specific fixation and embedding procedures for each individual sample.
• Thin sectioning of thin polymer films

Service and User charges

Additional equipment available:

• Glass knife maker (GKM, RMC, Boeckeler instruments).
• Ladd carbon evaporator
• Polymerization oven
• Darkroom

Attention students who wish to enroll in NSC 810 contact: Dr. Alicia Pastor, 517-353-5004, pastorle@msu.edu.. This is a limited enrollment course with specific enrollment procedures.